Modification:Article Title: SUMOylation Protects Endothelial Cell-Expressed Leukocyte-Specific Protein 1 from Ubiquitination-Mediated Proteasomal Degradation and Facilitates Its Nuclear Export.
Article Snippet: Human embryonic kidney 293T (HEK293T; Cat#CRL-3216) cells and the murine microvascular endothelial cell line SVEC4-10EE2 (Cat#CRL-2167) were obtained from the American Type Culture Collection (Manassas, VA, USA).. These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 ◦C and supplied with 5% CO2.These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 ◦C and..
Article Title: SUMOylation Protects Endothelial Cell-Expressed Leukocyte-Specific Protein 1 from Ubiquitination-Mediated Proteasomal Degradation and Facilitates Its Nuclear Export
Article Snippet: Human embryonic kidney 293T (HEK293T; Cat#CRL-3216) cells and the murine microvascular endothelial cell line SVEC4-10EE2 (Cat#CRL-2167) were obtained from the American Type Culture Collection (Manassas, VA, USA).. These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 °C and supplied with 5% CO 2 .These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 °C and..
Western Blot:Article Title: SUMOylation Protects Endothelial Cell-Expressed Leukocyte-Specific Protein 1 from Ubiquitination-Mediated Proteasomal Degradation and Facilitates Its Nuclear Export.
Article Snippet: Human embryonic kidney 293T (HEK293T; Cat#CRL-3216) cells and the murine microvascular endothelial cell line SVEC4-10EE2 (Cat#CRL-2167) were obtained from the American Type Culture Collection (Manassas, VA, USA).. These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 ◦C and supplied with 5% CO2.These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 ◦C and..
Article Title: SUMOylation Protects Endothelial Cell-Expressed Leukocyte-Specific Protein 1 from Ubiquitination-Mediated Proteasomal Degradation and Facilitates Its Nuclear Export
Article Snippet: Human embryonic kidney 293T (HEK293T; Cat#CRL-3216) cells and the murine microvascular endothelial cell line SVEC4-10EE2 (Cat#CRL-2167) were obtained from the American Type Culture Collection (Manassas, VA, USA).. These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 °C and supplied with 5% CO 2 .These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 °C and..
Expressing:Article Title: SUMOylation Protects Endothelial Cell-Expressed Leukocyte-Specific Protein 1 from Ubiquitination-Mediated Proteasomal Degradation and Facilitates Its Nuclear Export.
Article Snippet: Human embryonic kidney 293T (HEK293T; Cat#CRL-3216) cells and the murine microvascular endothelial cell line SVEC4-10EE2 (Cat#CRL-2167) were obtained from the American Type Culture Collection (Manassas, VA, USA).. These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 ◦C and supplied with 5% CO2.These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 ◦C and..
Article Title: SUMOylation Protects Endothelial Cell-Expressed Leukocyte-Specific Protein 1 from Ubiquitination-Mediated Proteasomal Degradation and Facilitates Its Nuclear Export
Article Snippet: Human embryonic kidney 293T (HEK293T; Cat#CRL-3216) cells and the murine microvascular endothelial cell line SVEC4-10EE2 (Cat#CRL-2167) were obtained from the American Type Culture Collection (Manassas, VA, USA).. These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 °C and supplied with 5% CO 2 .These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 °C and..
Isolation:Article Title: SUMOylation Protects Endothelial Cell-Expressed Leukocyte-Specific Protein 1 from Ubiquitination-Mediated Proteasomal Degradation and Facilitates Its Nuclear Export.
Article Snippet: Human embryonic kidney 293T (HEK293T; Cat#CRL-3216) cells and the murine microvascular endothelial cell line SVEC4-10EE2 (Cat#CRL-2167) were obtained from the American Type Culture Collection (Manassas, VA, USA).. These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 ◦C and supplied with 5% CO2.These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 ◦C and..
Article Title: SUMOylation Protects Endothelial Cell-Expressed Leukocyte-Specific Protein 1 from Ubiquitination-Mediated Proteasomal Degradation and Facilitates Its Nuclear Export
Article Snippet: Human embryonic kidney 293T (HEK293T; Cat#CRL-3216) cells and the murine microvascular endothelial cell line SVEC4-10EE2 (Cat#CRL-2167) were obtained from the American Type Culture Collection (Manassas, VA, USA).. These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 °C and supplied with 5% CO 2 .These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 °C and..
Conjugation Assay:Article Title: SUMOylation Protects Endothelial Cell-Expressed Leukocyte-Specific Protein 1 from Ubiquitination-Mediated Proteasomal Degradation and Facilitates Its Nuclear Export.
Article Snippet: Human embryonic kidney 293T (HEK293T; Cat#CRL-3216) cells and the murine microvascular endothelial cell line SVEC4-10EE2 (Cat#CRL-2167) were obtained from the American Type Culture Collection (Manassas, VA, USA).. These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 ◦C and supplied with 5% CO2.These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 ◦C and..
Article Title: SUMOylation Protects Endothelial Cell-Expressed Leukocyte-Specific Protein 1 from Ubiquitination-Mediated Proteasomal Degradation and Facilitates Its Nuclear Export
Article Snippet: Human embryonic kidney 293T (HEK293T; Cat#CRL-3216) cells and the murine microvascular endothelial cell line SVEC4-10EE2 (Cat#CRL-2167) were obtained from the American Type Culture Collection (Manassas, VA, USA).. These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 °C and supplied with 5% CO 2 .These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 °C and..
Translocation Assay:Article Title: SUMOylation Protects Endothelial Cell-Expressed Leukocyte-Specific Protein 1 from Ubiquitination-Mediated Proteasomal Degradation and Facilitates Its Nuclear Export.
Article Snippet: Human embryonic kidney 293T (HEK293T; Cat#CRL-3216) cells and the murine microvascular endothelial cell line SVEC4-10EE2 (Cat#CRL-2167) were obtained from the American Type Culture Collection (Manassas, VA, USA).. These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 ◦C and supplied with 5% CO2.These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 ◦C and..
Article Title: SUMOylation Protects Endothelial Cell-Expressed Leukocyte-Specific Protein 1 from Ubiquitination-Mediated Proteasomal Degradation and Facilitates Its Nuclear Export
Article Snippet: Human embryonic kidney 293T (HEK293T; Cat#CRL-3216) cells and the murine microvascular endothelial cell line SVEC4-10EE2 (Cat#CRL-2167) were obtained from the American Type Culture Collection (Manassas, VA, USA).. These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 °C and supplied with 5% CO 2 .These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 °C and..
Transfection:Article Title: SUMOylation Protects Endothelial Cell-Expressed Leukocyte-Specific Protein 1 from Ubiquitination-Mediated Proteasomal Degradation and Facilitates Its Nuclear Export.
Article Snippet: Human embryonic kidney 293T (HEK293T; Cat#CRL-3216) cells and the murine microvascular endothelial cell line SVEC4-10EE2 (Cat#CRL-2167) were obtained from the American Type Culture Collection (Manassas, VA, USA).. These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 ◦C and supplied with 5% CO2.These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 ◦C and..
Article Title: SUMOylation Protects Endothelial Cell-Expressed Leukocyte-Specific Protein 1 from Ubiquitination-Mediated Proteasomal Degradation and Facilitates Its Nuclear Export
Article Snippet: Human embryonic kidney 293T (HEK293T; Cat#CRL-3216) cells and the murine microvascular endothelial cell line SVEC4-10EE2 (Cat#CRL-2167) were obtained from the American Type Culture Collection (Manassas, VA, USA).. These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 °C and supplied with 5% CO 2 .These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 °C and..
Fractionation:Article Title: SUMOylation Protects Endothelial Cell-Expressed Leukocyte-Specific Protein 1 from Ubiquitination-Mediated Proteasomal Degradation and Facilitates Its Nuclear Export.
Article Snippet: Human embryonic kidney 293T (HEK293T; Cat#CRL-3216) cells and the murine microvascular endothelial cell line SVEC4-10EE2 (Cat#CRL-2167) were obtained from the American Type Culture Collection (Manassas, VA, USA).. These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 ◦C and supplied with 5% CO2.These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 ◦C and..
Article Title: SUMOylation Protects Endothelial Cell-Expressed Leukocyte-Specific Protein 1 from Ubiquitination-Mediated Proteasomal Degradation and Facilitates Its Nuclear Export
Article Snippet: Human embryonic kidney 293T (HEK293T; Cat#CRL-3216) cells and the murine microvascular endothelial cell line SVEC4-10EE2 (Cat#CRL-2167) were obtained from the American Type Culture Collection (Manassas, VA, USA).. These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 °C and supplied with 5% CO 2 .These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 °C and..
Membrane:Article Title: SUMOylation Protects Endothelial Cell-Expressed Leukocyte-Specific Protein 1 from Ubiquitination-Mediated Proteasomal Degradation and Facilitates Its Nuclear Export.
Article Snippet: Human embryonic kidney 293T (HEK293T; Cat#CRL-3216) cells and the murine microvascular endothelial cell line SVEC4-10EE2 (Cat#CRL-2167) were obtained from the American Type Culture Collection (Manassas, VA, USA).. These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 ◦C and supplied with 5% CO2.These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 ◦C and..
Article Title: SUMOylation Protects Endothelial Cell-Expressed Leukocyte-Specific Protein 1 from Ubiquitination-Mediated Proteasomal Degradation and Facilitates Its Nuclear Export
Article Snippet: Human embryonic kidney 293T (HEK293T; Cat#CRL-3216) cells and the murine microvascular endothelial cell line SVEC4-10EE2 (Cat#CRL-2167) were obtained from the American Type Culture Collection (Manassas, VA, USA).. These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 °C and supplied with 5% CO 2 .These cells were cultured in Dulbecco’s Modified Eagle’s Medium (Corning, Tewksbury, MA, USA; Cat#10013CV), supplemented with 10% fetal bovine serum (Cytiva Hyclone, Marlborough, MA, USA; Cat#SH30071.03HI), penicillin-streptomycin (100 units/mL and 100 μg/mL, respectively; Gibco, Waltham, MA, USA; Cat#15140122) in a cell culture incubator set at 37 °C and..
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